Method agreement between three different chambers for comparative bull semen computer assisted sperm motility analysis

The aim of this study was to compare computer assisted sperm analysis (CASA) results frozen thawed bull semen using three different chambers. Sixty samples were (37°C; 30 sec), extended in PBS (30×106 spermatozoa/mL; 37°C) and incubated 2 min). Each sample analyzed by CASA [total motility, progressive (pro)/ non-progressive/rapid/medium/slow movement spermatozoa, VCL, VSL, VAP, ALH, BCF, LIN, STR, WOB hyperactive spermatozoa] chambers: a Makler® chamber (MC; 10 μm); Leja 4 slide (LC; 20 Glass covered with coverslip (GSC; 10.3 μm). Makler gave higher values compared both the LC GSC for almost all examined parameters. No systematic effect evident between WOB, BCF. Method agreement MC generally moderate, poor moderate good. In general, narrower limits found lower values. conclusion, outcomes could be influenced This finding should taken into consideration when comparing from laboratories.